The Chacala Trail is popular with horseback riders. It consists of a series of loops encompassing 6.5 miles of level path. The trail received its name from a small freshwater pond just outside the Preserve boundary. Several distinct biological communities including pine flatwoods, hardwood hammock, baygall, and open ponds are seen. Wildlife viewing is supreme along this trail, especially for the quiet visitor. Common sights include white-tailed deer, bobcat, wild turkey, pileated woodpecker, and bald eagle. The trail does permit foot traffic and mountain bikers.

Directions: From Gainesville, FL, Travel south on Interstate 75. Take exit 75 heading east to U.S. Highway 441. Travel north a short distance to reach the preserve. Look for signage near the main entrance. Parking available.

Chacala Trail is named after a small freshwater pond just outside the preserve boundary.